George Rice

Fluorescent Microscopy part of Microbial Life:Research Methods:Microscopy
Educational webpage detailing fluorescent microscopy principles, operation, and biological applications, including epi-fluorescence and confocal techniques, with diagrams and literature references, part of a microbial research methods module authored by George Rice.

Fluorescent In Situ Hybridization: (FISH) part of Microbial Life:Research Methods:Microscopy
Educational webpage explaining Fluorescent In Situ Hybridization (FISH) as a molecular microbiology technique, detailing its mechanism, applications in microbial identification and gene expression analysis, and including visual examples of bacterial and archaeal differentiation using fluorescent probes.

Three Dimensional Phylogenetic Relationships: part of Microbial Life:Research Methods
This educational webpage presents a microbiology lab exercise on visualizing phylogenetic relationships in three dimensions, detailing a protocol using SDSC Biology WorkBench and MATLAB to transform ClustalW distance matrices into 3D trees, authored by George Rice and Gretchen A. Koch as part of the Microbial Life: Research Methods module.

Selecting a Protein Data Set: part of Microbial Life:Research Methods
Instructional guide detailing how to select and analyze protein data sets using Biology WorkBench, covering account creation, sequence retrieval via Ndjinn, alignment with CLUSTALW, phylogenetic tree generation, and distance matrix export to MATLAB for advanced analysis.

Replication:DNA copied into DNA part of Microbial Life:Research Methods:Genomics
Educational webpage detailing DNA replication in microbial genomics, covering the molecular process of DNA synthesis, replication fork formation, enzyme functions (helicases, polymerases, topoisomerases), and complementary strand assembly, with links to animations and teaching resources for undergraduate and graduate education.

Methods:Mammoth Hot Springs part of Microbial Life:Research Methods:Bio-geochemical Methods
A scientific methods page detailing interdisciplinary research at Mammoth Hot Springs, covering in situ crystallization experiments, microbial community analysis, and stochastic modeling of carbonate terrace formation through biogeochemical and geological techniques.

Translation: Messenger RNA Translated Into Protein part of Microbial Life:Research Methods:Genomics
Educational page detailing the molecular biology process of translation, where mRNA is decoded by ribosomes to synthesize proteins, covering mechanisms, RNA types (mRNA, tRNA, rRNA), step-by-step protein synthesis, and related teaching resources within a genomics curriculum.

Polymerase Chain Reaction: (PCR) part of Microbial Life:Research Methods:Genomics
Educational content page detailing the polymerase chain reaction (PCR) technique, covering its principles, applications in genomics and molecular biology, instrumentation like thermocyclers, and teaching resources, within the context of microbial research methods.

Ribosomes and Ribosomal RNA:(rRNA) part of Microbial Life:Research Methods:Genomics
Educational webpage detailing ribosomes and ribosomal RNA (rRNA), emphasizing their role in protein synthesis and the significance of the 16S rRNA gene in microbial genomics, including structural features, functional mechanisms, and applications in modern genomics research.

Molecular Finger Printing part of Microbial Life:Research Methods:Genomics
This educational webpage explains molecular fingerprinting techniques for microbe identification, detailing steps like environmental sampling, DNA extraction, PCR amplification of 16S rRNA genes, cloning, sequencing, and bioinformatic analysis using tools like BLAST, within the context of microbial genomics and ecology research.

Modern Genomics:How to identify microbial species. part of Microbial Life:Research Methods:Genomics
Educational content page covering microbial genomics, focusing on 16S rRNA gene sequencing for species identification, phylogenetic analysis, and the limitations of traditional culturing methods, featuring contributions by George Rice and references to foundational work by Carl Woese and Norman Pace.

Replication: DNA copied into DNA part of Microbial Life:Research Methods:Genomics
Educational webpage detailing DNA replication and transcription processes in genomics, featuring step-by-step molecular mechanisms, RNA types (mRNA, tRNA, rRNA), and their roles in genetic expression within microbial life research.

DNA Extraction part of Microbial Life:Research Methods:Genomics
Educational webpage detailing DNA extraction techniques in molecular biology and genomics, covering principles, laboratory methods, instrumentation (e.g., bead beater, centrifuge, gel electrophoresis), and teaching activities for K–12 and undergraduate levels, authored by George Rice and hosted by SERC.

SMaRT Course2006 part of Microbial Life:Microbial Observatories:Marine Sponges MO
Informational webpage detailing the 2006 SMaRT course, an NSF-funded, intensive undergraduate training program in molecular microbial ecology focused on marine sponges, hosted at the University of Maryland Biotechnology Institute, featuring participant and instructor profiles, research activities, and project outcomes.

Results:Mammoth Hot Springs part of Microbial Life:Research Methods:Bio-geochemical Methods
Research Methods page detailing biogeochemical analysis of Mammoth Hot Springs, covering fluid dynamics, microbial influence on carbonate precipitation, bacterial community partitioning, and isotopic fractionation studies using molecular techniques and lipid biomarkers.

Geobiology and the Emergence of Terraced Architecture during Carbonate Mineralization : Mammoth, Yellowstone National Park part of Microbial Life:Research Methods:Bio-geochemical Methods
A research methods page exploring geobiology and microbe-mediated carbonate mineralization at Mammoth Hot Springs, Yellowstone, detailing interdisciplinary studies on terraced architecture formation through microbial activity, geochemistry, and modeling.

Project Significance: part of Microbial Life:Research Methods:Bio-geochemical Methods
A content page detailing the scientific significance of microbial research at Mammoth Hot Springs, focusing on biogeochemical methods, terraced carbonate architecture, and microbial influence on geological formations, part of a larger educational resource on microbial life research techniques.

Are Viruses Alive? part of Microbial Life:Topics of Interest:Yellowstone Thermal Viruses
Content page exploring whether viruses are alive, covering their biological classification, structure, replication, evolutionary role, and dominance in biomass, with a focus on Yellowstone thermal viruses and related teaching activities.

2004 Sampling Field Trip: part of Microbial Life:Microbial Observatories:Nevada Hot Springs MO
Content page detailing a 2004 field trip to 16 Nevada hot springs, covering microbial diversity, biogeochemical function, and geothermal biogeography in Great Basin valleys, with sample collection, researcher interactions, and photographic documentation.

Project Significance part of Microbial Life:Microbial Observatories:Sapelo Island MO
Content page detailing the scientific significance of the Sapelo Island Microbial Observatory project, focusing on microbial transformations of dimethylsulfoniopropionate (DMSP) and dimethylsulfide (DMS) in salt marshes, their role in global sulfur flux and climate regulation, and associated research methods and findings.

Project Significance: part of Microbial Life:Microbial Observatories:Monterey Bay MO
Content page from the Microbial Life Education Resource discussing the significance of marine microbial communities in global nutrient cycling, emphasizing their role in carbon, nitrogen, and phosphorus dynamics within ocean ecosystems, particularly in Monterey Bay, and highlighting the application of metagenomics to study microbial diversity and function in situ.

Methods: Monterey Bay MO part of Microbial Life:Microbial Observatories:Monterey Bay MO
Content page detailing molecular research methodologies in the Monterey Bay Microbial Observatory, focusing on BAC library construction, DNA microarray development, and genomic analysis techniques for studying marine microbial communities.

Environment: Monterey Bay part of Microbial Life:Microbial Observatories:Monterey Bay MO
Scientific webpage detailing microbial ecology research in Monterey Bay, featuring environmental genomic libraries, phylogenetic reconstructions of uncultured marine microbes (e.g., γ-Proteobacteria, Roseobacter), and oceanographic data from CTD cruises, with downloadable figures and methods from the DeLong Lab.

Methods part of Microbial Life:Microbial Observatories:Sapelo Island MO
Content page detailing research methods used in the Sapelo Island Microbial Observatory (SIMO) project, focusing on flow cytometry analysis for studying prokaryotic assemblages in salt marsh ecosystems to advance understanding of microbial processes and ecosystem regulation.

Results:SIMO part of Microbial Life:Microbial Observatories:Sapelo Island MO
This results page from the Sapelo Island Microbial Observatory (SIMO) project details research findings on microbial diversity in coastal marsh ecosystems using 16S rRNA sequencing, including development of an environmental sequence database, automated taxonomic analysis tools (RDPquery), statistical methods for clone library comparison (LIBSHUFF), flow cytometry-based population sorting, and collaborative environmental transcriptomics with implications for nutrient cycling and gene expression studies.

Project Significance (The Importance of Carbon): part of Microbial Life:Microbial Observatories:Oligotrophic Ocean MO
Created by George Rice, Montana State University "Carbon is what we (as well as all of the other plants and animals on earth) are made of (50% of our dry weight). Ecologists can learn a lot about ecosystems ...

Bermuda Atlantic Time-Series Study (BATS) Site: part of Microbial Life:Microbial Observatories:Oligotrophic Ocean MO
Created by George Rice, Montana State University The BATS site is characterized as being seasonally oligotrophic, and significantly influenced by regular annual patterns of temperature variability and mixing of ...

Methods employed at the Microbial Observatory: part of Microbial Life:Microbial Observatories:Oligotrophic Ocean MO
Created by George Rice, Montana State University The Oceanic Microbial Observatory combines oceanographic microbial ecological methodology with molecular microbiological /ecological expertise to elucidate the ...

Findings: part of Microbial Life:Microbial Observatories:Oligotrophic Ocean MO
Created by George Rice, Montana State University (click on images for larger version)  The northwestern Sargasso Sea displays seasonal dynamics in DOC accumulation and distribution of biomass as well ...

McMurdo Dry Valleys:Principal Investigators part of Microbial Life:Microbial Observatories:McMurdo MO
A content page listing principal investigators of the McMurdo Dry Valleys Microbial Observatory project, featuring bios and contact details for John C. Priscu, Brian D. Lanoil, Michael T. Madigan, and Stephen J. Giovannoni, within a microbial life educational resource site.

RLMO Education and Outreach part of Microbial Life:Microbial Observatories:Red Layer
This educational webpage details the RLMO (Red Layer Microbial Observatory) project's outreach initiatives, offering undergraduate and secondary-level students hands-on research experiences in microbial diversity, DNA analysis, and computational biology using Yellowstone hot spring bacteria, with curriculum resources and partnerships supporting STEM education.

Culturing Cold Prokaryotes: part of Microbial Life:Microbial Observatories:McMurdo MO
Content page from the Microbial Life collection detailing cold prokaryote culturing methods in McMurdo Dry Valleys lakes, covering nutrient cycle research (C, N, S), high-throughput and conventional culturing techniques, molecular phylogeny, and fluorescence in situ hybridization (FISH) applications in extreme Antarctic environments.

Red Layer Microbial Observatory (RLMO):Phase 1 and 2 part of Microbial Life:Microbial Observatories:Red Layer
Content page detailing the Red Layer Microbial Observatory (RLMO) project's first two phases, covering research goals, results, and educational outreach focused on thermophilic Chloroflexi bacteria in Yellowstone's hydrothermal environments, including genetic analysis, environmental distribution, and implications for early photosynthesis.

Project Significance: part of Microbial Life:Microbial Observatories:Marine Sponges MO
This educational webpage details a Microbial Observatories project investigating microbial diversity in marine sponges, covering research significance, methods like 16S rRNA analysis and fluorescent in situ hybridization, and potential applications in bioactive compound discovery and bacterial signaling pathways.

McMurdo Dry Valleys MO:Experimental Approach part of Microbial Life:Microbial Observatories:McMurdo MO
Content page detailing the experimental approach of the McMurdo Dry Valleys Microbial Observatory project, covering multidisciplinary research methods, ice drilling, water and sediment sampling, site characterization, and principal investigators involved in studying microbial life in extreme Antarctic environments.

Indonesia Sampling Trip(December 05) part of Microbial Life:Microbial Observatories:Marine Sponges MO
Content page detailing a December 2005 Indonesia sampling trip led by PI Russell Hill and graduate student Naomi Montalvo, focusing on collecting Xestospongia testudinaria sponges in Manado for microbial community analysis, supported by NSF's Microbial Observatories program.

Alkalithermophilic Chemolithoautotrophic Crenarchaeota part of Microbial Life:Microbial Observatories:Nevada Hot Springs MO
Content Page detailing alkalithermophilic chemolithoautotrophic Crenarchaeota in Nevada hot springs, covering microbial adaptations to extreme alkaline and thermal conditions, metabolic processes using inorganic energy sources, and phylogenetic placement within the Archaea domain.

Outreach: part of Microbial Life:Microbial Observatories:Marine Sponges MO
Content Page detailing the outreach component of a Microbial Observatories project focused on marine sponges, highlighting the Summer Microbiology and Research Training (SMaRT) course, undergraduate research engagement, laboratory methods in microbial ecology, and broader scientific questions about symbiotic bacteria in marine invertebrates.

Project Significance: part of Microbial Life:Microbial Observatories:Contaminated Aquifer MO
A content page detailing the significance of a microbial observatory project studying naphthalene degradation in a coal tar-contaminated aquifer, highlighting its biogeochemical, genetic, and environmental remediation implications.

Results:Contaminated Aquifer MO part of Microbial Life:Microbial Observatories:Contaminated Aquifer MO
Results page from a microbial observatory project on a contaminated aquifer, detailing molecular and field-based findings on naphthalene-degrading bacteria, biodegradation processes, gene expression, microbial diversity, and horizontal gene transfer, with links to publications and related research.

Course Announcement (PowerPoint 2.2MB Jan5 06) (Summer 2006): part of Microbial Life:Microbial Observatories:Marine Sponges MO
This informational webpage announces a 2006 NSF-funded undergraduate training program (SMaRT) focused on microbial ecology in marine sponges, detailing course content, application process, faculty, and logistical information for students from underrepresented groups, hosted by SERC as part of its Microbial Observatories initiative.

2005 Sampling Field Trip:3 Days in the Desert part of Microbial Life:Microbial Observatories:Nevada Hot Springs MO
A content page detailing a 2005 three-day field sampling trip to Nevada hot springs, documenting geothermal sites like Eagleville and Surprise Valley with temperature, pH, and photographic records, part of the Microbial Observatories project under the SERC website.

Publications part of Microbial Life:Microbial Observatories:Contaminated Aquifer MO
A content page listing scientific publications related to microbial ecology and biodegradation in a coal tar waste-contaminated aquifer, featuring research on naphthalene dioxygenase genes, horizontal gene transfer, and microbial life in contaminated environments, authored by George Rice and colleagues.

Results: part of Microbial Life:Microbial Observatories:Marine Sponges MO
Content page from the Microbial Life collection presenting research results on marine sponge microbiomes, detailing microbial community analyses of Key Largo and Indonesian sponges, novel actinobacteria discoveries, quorum sensing, and proteomic studies, with links to related methods, publications, and outreach.

NIMO Course of Study part of Microbial Life:Microbial Observatories:North Inlet MO
A scientific results page from the North Inlet Microbial Observatory project detailing research methods and findings on diazotrophs in salt marsh ecosystems, including nitrogen fixation studies, nifH gene analysis, and microbial community profiling via DGGE, with associated image metadata and reuse information.

McMurdo Dry Valleys: Water Sample Analysis part of Microbial Life:Microbial Observatories:McMurdo MO
Content page detailing water sample analysis in Antarctica's McMurdo Dry Valleys, focusing on biogeochemical methods like the Winkler technique for measuring dissolved oxygen, using Niskin bottles for depth-specific lake water collection, and presenting chemical profiles from lakes such as Fryxell.

The Nitrogen Cycle: part of Microbial Life:Microbial Observatories:North Inlet MO
Educational webpage detailing the nitrogen cycle with emphasis on diazotrophs—nitrogen-fixing bacteria—in salt marsh ecosystems, featuring the North Inlet Microbial Observatory (NIMO) project, its research goals, team, and microbial ecology context.

Viruses found in Yellowstone: part of Microbial Life:Topics of Interest:Yellowstone Thermal Viruses
Educational page detailing thermophilic viruses in Yellowstone, including TEM images and descriptions of viral morphologies like Fuselloviridae, Rudiviridae, Lipothrixviridae, and the Sulfolobus Turreted Icosahedral Virus (STIV), with research methods and contextual information on extremophiles.

Culturing Viral Host Organisms: part of Microbial Life:Topics of Interest:Yellowstone Thermal Viruses
This educational webpage details aerobic and anaerobic culturing techniques for thermophilic microbial hosts of viruses in Yellowstone, featuring lab methods, equipment, and environmental adaptations, authored by George Rice for the Microbial Life: Yellowstone Thermal Viruses module.

Experimental Approach: part of Microbial Life:Topics of Interest:Yellowstone Thermal Viruses
This educational webpage details the experimental methodology for studying thermal viruses in Yellowstone National Park, covering site identification, sterile sampling techniques, culturing host organisms, virus detection via transmission electron microscopy, and characterization of isolated viruses, within the context of extremophile microbiology research.

Thermal Virus Hosts in Yellowstone part of Microbial Life:Topics of Interest:Yellowstone Thermal Viruses
Content page detailing archaeal hosts of thermal viruses in Yellowstone National Park, featuring TEM images and biological descriptions of Acidianus, Thermofilum, and Thermoproteus, including their morphology, metabolic traits, and viral interactions.

Sulfolobus: part of Microbial Life:Topics of Interest:Yellowstone Thermal Viruses
Content page from the Microbial Life collection discussing *Sulfolobus*, a thermophilic archaeon found in Yellowstone's extreme environments, highlighting its role as a host for SSV viruses, laboratory cultivation advantages, sulfur metabolism, and genomic research, with supporting TEM images and contextual links to related topics.

Environmental Sampling part of Microbial Life:Research Methods:Environmental Sampling
Educational resource page detailing environmental sampling techniques in microbiological research, covering methods for measuring pH, electrical conductivity, dissolved oxygen (via Winkler method), turbidity (Secchi disk), stream gaging, hydrological tracers, and stable isotopes, with supporting images and links to specialized protocols.

Genomics: ( genetics - genes - genetic code - RNA/DNA) part of Microbial Life:Research Methods:Genomics
Educational page introducing genomics as a biotechnology field focused on genetics, DNA/RNA, and molecular biology techniques, covering core concepts like the central dogma (replication, transcription, translation), DNA structure, and related teaching resources within microbial life research methods.

MLER Methods: part of Microbial Life:Research Methods
Educational webpage offering a comprehensive novice guide to microbiology research techniques, covering environmental sampling, biogeochemical methods, genomics, microscopy, and integrated research case studies, designed to enhance scientific literacy and research implementation.

A Genomics-enabled Microbial Observatory in the Monterey Bay National Marine Sanctuary part of Microbial Life:Microbial Observatories:Monterey Bay MO
A scientific overview page detailing a genomics-enabled microbial observatory in Monterey Bay National Marine Sanctuary, focusing on microbial ecology research goals, genomic technologies, marine microbiology, and the work of PI Edward F. DeLong, part of the Microbial Observatories educational resource.

Dynamics of Prokaryotic Communities in a Southeastern U.S. Salt Marsh part of Microbial Life:Microbial Observatories:Sapelo Island MO
A scientific educational webpage detailing the Sapelo Island Microbial Observatory's research on prokaryotic community dynamics in a southeastern U.S. salt marsh, covering project background, principal investigators, microbial diversity, and biogeochemical processes within a coastal estuarine ecosystem.

M.O. Title: An Oligotrophic Oceanic Microbial Observatory part of Microbial Life:Microbial Observatories:Oligotrophic Ocean MO
Created by George Rice, Montana State University This project focuses geographically on the Bermuda Atlantic Time-series Study (BATS) site. This site is one of the longest time-series studies of oceanographic ...

The Red Layer Microbial Observatory—a Research Experience for Undergraduates in Yellowstone part of Microbial Life:Microbial Observatories:Red Layer
A project page detailing the Red Layer Microbial Observatory, an undergraduate research initiative in Yellowstone National Park focused on studying extremophilic microbial communities in thermal springs, with emphasis on molecular diversity, stromatolite analogs, and long-term ecological monitoring across designated field sites.

M.O. Title: Diversity, function, and biogeochemical consequences of chemolithoautotrophic Archaea in Nevada hot springs part of Microbial Life:Microbial Observatories:Nevada Hot Springs MO
A project page detailing a Microbial Observatories study on alkalithermophilic chemolithoautotrophic Crenarchaeota in Nevada hot springs, covering their diversity, CO2 fixation functions, biogeochemical roles, and comparative analysis with other geothermal sites, while highlighting principal investigators and educational goals.

M.O. Title:Microbial Diversity of Prokaryotes in Marine Sponges of the Class Demospongiae part of Microbial Life:Microbial Observatories:Marine Sponges MO
Project webpage detailing a microbial observatory study on prokaryotic diversity within marine sponges (Demospongiae) at Conch Reef, Florida, focusing on symbiotic bacteria, bioactive compound potential, and molecular analysis methods, led by principal investigators Russell Hill, Clay Fuqua, and Feng Chen.

Collaborative Research: Microbial Diversity and Function in the Permanently Ice-Covered Lakes of the McMurdo Dry Valleys, Antarctica part of Microbial Life:Microbial Observatories:McMurdo MO
This educational webpage details a microbial ecology research project focused on microbial diversity and function in the permanently ice-covered lakes of Antarctica's McMurdo Dry Valleys, covering experimental approaches, sample analysis, culturing techniques, and integration with the Long-Term Ecological Research (LTER) Network.

Mo Title: North Inlet Microbial Observatory (NIMO) part of Microbial Life:Microbial Observatories:North Inlet MO
A project page detailing the North Inlet Microbial Observatory (NIMO), focusing on diazotrophic bacteria in a South Carolina salt marsh ecosystem, outlines research goals, principal investigators, and microbial diversity studies along environmental gradients.

M.O. Title: Observing Microbial Diversity and Horizontal Gene Transfer In A Shallow Contaminated Aquifer part of Microbial Life:Microbial Observatories:Contaminated Aquifer MO
Project webpage detailing microbial diversity and horizontal gene transfer research in a naphthalene-contaminated aquifer at South Glens Falls, NY, led by Dr. Eugene L. Madsen and Dr. William C. Ghiorse, covering research goals, site background, and team information.

Microbial Observatories (MO) part of Microbial Life:Microbial Observatories
Informational page profiling NSF-funded Microbial Observatories projects, detailing research on microbial diversity, consortia, and ecological processes in extreme environments, with educational outreach and data dissemination through an internet-accessible network.

Looking For Thermal Viruses in Yellowstone National Park part of Microbial Life:Topics of Interest:Yellowstone Thermal Viruses
This educational webpage from SERC's Microbial Life collection explores the discovery and study of thermal viruses in Yellowstone National Park's geothermal features, detailing their significance in extreme environment research, biotech applications, and molecular adaptations of Archaeal hyper-thermophiles, while providing an experimental overview and teaching resources on viral life characteristics.