Design2Data
Ashley Vater, University of California-Davis
The D2D program is centered around an undergraduate-friendly protocol workflow that follows the design-build-test-learn engineering framework. This protocol has served as the scaffold for a successful undergraduate training program and has been further developed into courses that range from a 10-week freshman seminar to a year-long, upper-division molecular biology course. The overarching research goal of this CURE probes the current predictive limitations of protein-modeling software by functionally characterizing single amino acid mutants in a robust model system. The most interesting outcomes of this project are dependent on large datasets, and, as such, the project is optimal for multi-institutional collaborations.

Random gene mutagenesis for gene identification linked to prodigiosin production in Serratia marcescens
Verena Carvalho, University of Massachusetts-Amherst
This lab course is designed to provide course-based undergraduate research experiences. You will learn how to prepare, execute, and interpret your own experiments. While all of you will conduct the same techniques in the course, each of you will create their own sets of mutant strains and study different features of your bacterium. We will study Serratia marcescens, an opportunistic, nosocomial pathogen, and is particularly linked to catheter-associated bacteremia, urinary tract infections, and wound infections. It is responsible for 1.4% of hospital-acquired infection cases in the United States. These bacteria are commonly found in the respiratory and urinary tracts of hospitalized adults, and in the gastrointestinal systems of children. Many strains of S. marcescens have a bright red colony color (a tripyrrole pigment called prodigiosin), while pigment production is often temperature-dependent. Prodigiosin is a secondary metabolite, and its expression is thought to be related to phosphate limitation. It was also identified as a natural bioactive substance with high potential for antibiotic and anti-cancer applications. It currently receives renewed attention for its wide range of potential applications, including activities as antimalarial, antifungal, immunosuppressant, and antibiotic agents. It is also prominently known for its capacity to trigger apoptosis of malignant cancer cells, and high activity against stationary phase Borrelia burgdorferi, the causative agent of Lyme disease, has been demonstrated. Given its diverse effects, the exact mechanisms are currently not elucidated, and may be highly complex, including phosphatase inhibition, copper mediated cleavage of double stranded DNA, or disrupting the pH gradient through transmembrane transport of H+ and Cl- ions. Clearly, prodigiosin is a highly promising drug candidate, and is currently in preclinical phase study for pancreatic cancer treatment. In this course, we will use the transposon Tn5 to generate random mutations in the chromosome of Serratia marcescens. The transposon will be provided by a plasmid hosted in a donor E. coli strain, and transferred into your test bacterium via conjugation. We will then first select for successfully transposed mutants by testing for antibiotic resistance, and screen for your mutants that are altered in their pigment production. To identify the gene where the mutation has happened, we will remove the chromosomal DNA from the mutant strains, perform restriction enzyme digest, and generate self-circulating DNA. These plasmids are transformed into an E. coli strain that can replicate the fragment of genomic DNA that contains the transposon, and we can sequence the insertion site with the transposon DNA as anchor. In summary, in this course you will gain hands-on experience with modern genetic and biotechnological techniques, you will gain insights into bioinformatics and into working with public databases, which are all essential skills in modern microbiological research.

Investigating local climate change impacts in a STEM first year learning community
Mara Brady, California State University-Fresno
still in progress...

Introduction to GIS using CURE
Elizabeth Bollen, Adams State University; Chayan Lahiri, Adams State University
Geographic information systems (GIS) are used in many capacities across every discipline to help communities, governments, and businesses make informed decisions. This Introduction to GIS CURE course aims to assist local ranches by locating sampling sites for watershed analyses. Students in this GIS course will develop skills manipulating data by learning GIS software. The students themselves are tasked with identifying sampling site criteria for the broader study, which will assist other student researchers in locating scientifically viable, safe, and accessible sampling sites for watershed sample collection. Students in this course will also use knowledge gained in prior classes to make and defend decisions. Written reports, figure drafting, and group discussions will help students learn how to clearly and effectively communicate their findings and results. The knowledge and skills students' gain in this course will be used in future classes and are highly sought after by employers.

Spatial Distribution of Food Resources in the Phoenix Metro Area.
Elena Ortiz, Phoenix College
The main research question/ driving question is: How are food resources distributed across the urban landscape? Students will explore previous work on food deserts in the Phoenix Metro area, use census data, USDA data, collect and compile data on the local food system, create data visualizations and maps, and determine future data needs. Students will also determine stakeholders and community members that could act on the results of their findings and identify appropriate ways to communicate their research to those audiences.

CURE: Overcoming seed dormancy in Cleome, a potential C4 model plant
Margaret Young, Elizabeth City State University

Animal Genome to Phenome - A CURE for food security
Mulumebet Worku, North Carolina A & T State University