Eyes in the Sky II > Index > ImageJ Techniques

ImageJ Index

ImageJ Concepts, Skills, & Techniques


Color
Digital images
Digital image - a digital image is a grid of numerical valuesusually measurementsdisplayed according to a lookup table or RGB color values.

Integrating ImageJ with other applications
Introduction to ImageJ

Measurement
Density Calibration - Density calibration involves relating pixel values to real world measurements, such as brightness, elevation, temperature, concentration, etc.
Density Measurements - Density measurements include statistical measures of pixel values, such as mean, median, and mode. If an image is density calibrated in some units, the measurements are given in calibrated units. If a region of interest (ROI) has been selected, only the pixels in the ROI is measured. If no ROI is defined, all of the pixel values in the image are considered.
Spatial Calibration ("Setting Scale") - Spatial calibration involves relating image distances, areas, and volumes in pixels to real world distances, areas, and volumes. Also referred to as setting scale.
Spatial Measurements - Spatial measurements include distances, areas, and volumes. If an image is uncalibrated, these are given in pixels, square pixels, and cubic pixels. If an image is spatially calibrated in some units, the measurements are given in units, square units, and cubic units. If a region of interest (ROI) has been selected, only the ROI is measured. If no ROI is defined, the entire image is measured.

Stacks
Stack - an image window containing two or more digital images as layers or slices. A normal image window has two dimensionswidth and height; a stack window has three dimensionswidth, height, and slices. Different slices typically represent differences or changes in time, space, wavelength, focal plane, or feature type.
Definition Stack basics Uses of stacks
Tools and techniques

ImageJ Resources




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