Exploring eukaryotic protein structure and post-translational modifications.
Erica Jacobs, St. John's University-New York
This CURE will provide opportunity for students to think and act as researchers by using computational, biochemical, and bioanalytical techniques to examine tick antigen proteins. The CURE is designed as a lab for upper-level students who are taking or have taken a one-semester introductory biochemistry course, but two semesters would be even better. It could also be adapted for cell/molecular biology or (bio) analytical chemistry instrumentational analysis labs. It has been taught for classes ranging from 12-24 students. Ticks are notorious vectors of viral, protozoan, and bacterial diseases, including Lyme disease. While an anti-vector vaccine capable of protecting people from diseases transmitted by a particular tick species is an alluring goal, only one such anti-tick vaccine is currently available. This vaccine targets Bm86, a protein from the midgut of Rhipicephalus microplus, a cattle tick. Not only does the vaccine limit parasitism of the cattle by ticks, data suggests that it can also prevent transmission of tick-borne diseases including bovine anaplasmosis and babesiosis. However, similar vaccination approaches have not succeeded thus far against ticks that transmit diseases to humans, and little is known about the antibody response to the antigen, or about the protein itself. Since the protein's structure and function are unknown, the research goal of this CURE is to purify Bm86 using an insect cell/baculovirus expression system and characterize it, including domain structure and post-translational modifications (glycosylation sites). There are homologs to Bm86 in every sequenced tick species examined, and future CUREs will characterize some of the homologs including those in Ixodes scapularis, the tick that is mainly responsible for transmitting Lyme in the eastern US, and Haemaphysalis longicornis, the Asian longhorned tick, a newly-discovered invasive species in the area that also has significant disease-transmitting potential. By understanding the structure and post-translational modifications of this protein, we hope to gain a better understanding of how to make effective anti-tick vaccines, including those for humans, that may prevent transmission of Lyme disease. Importantly, the basic parameters of this CURE can be used to examine other proteins besides tick antigens. For example, during the pandemic, the CURE pivoted from the tick antigen to the SARS-CoV-2 nucleocapsid protein, which was also expressed in an insect cell system. Instead of characterizing glycosylation sites, we characterized phosphorylation sites. It's therefore possible to use this same framework for many different eukaryotic proteins that may be of research interest.

Testing the reliability of various miRNA-scanning tools to predict and establish novel miRNAs and their function in Musa sp.
Supriyo Ray, Bowie State University
Bananas (Musa sp.) are one if the world's most important fruit and forms a staple food especially for tropical and subtropical countries. It provides food security to millions of people and an important contributor to their economy. miRNAs are non-coding small RNAs that help regulate many biological processes by degrading mRNAs and stopping their expression to proteins. miRNA libraries have been well characterized in animal cells while, there is much work left to be done for plants especially bananas. Few miRNAs have been identified for bananas and their regulation have been associated with external stimuli such as infection, stress, resistance etc. yet, lot of work needs to be done to develop a comprehensive library for banana cultivars. In this CURE program the students would participate and do research to predict and establish novel miRNAs in banana cultivars.

Identification of transcriptional factors linked to resistance to drought stressors in Musa accessions for multiplication and maintenance using tissue culture techniques
David Igwe, Bowie State University
Musa species are the favorite fruit crops of the world and their genotypes derived from M. acuminata (AA) and M. balbisiana (BB) exist and are highly polyploidy. They are challenged by drought effect that adversely impacts its growth, productivity and yield. Interestingly, genotypes with "B" genome (in particular ABB type) are more tolerant to abiotic stresses than those solely possessing "A" genome. Drought responses are notoriously multigenic and quantitative with strong environmental effects on genotypes. Transcription factors (TFs) are major players in drought stress signaling and generally constitute major portion of transcriptionally active regions in banana genome to act as central domain for drought signaling networks. Overexpression of some of drought stress-responsive TFs constitute effects of drought tolerance and resistance. The overall goal of this project is to assess transcriptional factors responsible for phenotypic expressions of resistance of Musa accessions to drought stressor for mass production and continuous maintenance of the selected accessions for research and CURE program.

Aging through Genes
Tyesha Burks, Bowie State University

Genetic Diversity and phylogenetic studies of Mushrooms in the Bowie State University Campus
George Ude, Bowie State University
The goal of my CURE is to have students be able to study biodiversity and genetic relationships among species. Students will utilize DNA barcoding techniques and other DNA fingerprinting techniques to study mushroom collections from Bowie State University ecosystem.

Elucidation of the mechanism involved in the cyto-protective effect of creatine in oxidative stress.
Lucia Santacruz, Bowie State University

I4: Incorporating Immunology in Introductory Biology to STEM majors
Devyn Gillette, Bowie State University
This CURE will utilize common techniques found in immunology to instruct first-year introductory biology students on how to conduct and answer biological research questions.

CRISPR-Cas9, Pancreatic Cancer, and Science Identity
Latanya Hammonds-Odie, Georgia Gwinnett College
At GGC, we have a history of providing all biology majors with meaningful laboratory experiences. For example, in our Cell Biology course that all majors are required to take, students learn to culture mammalian cells and design their own experiments using these cells. We plan to extend the in silico research of students into "wet bench" investigations for another course. Each student group would develop a rationale for their selection of gene(s) based on differential expression and on the current human pancreatic cancer research literature. They would design and implement a CRISPR-Cas9 deletion strategy to reduce specific gene expression that would be confirmed using immunoblotting and/or immunocytochemistry. Students would measure the proliferation, migration, and apoptosis of the control and the modified pancreatic cancer cells to assess the impact of their genetic manipulation. Harnessing the power of next-generation sequencing analysis with the ability to target specific genes for a reduction in expression will add to the knowledge base in the field; while allowing students to perform techniques that they have only explored in a lecture setting. For the students, the degree of ownership in the project, the stated confidence in the ability to "think like a scientist" and the ability to perform specific techniques should increase to a level comparable to a one-on-one mentored research experience.

Isolation and Identification of chloroplast lipid mutant from Arabidopsis
Jinjie Liu, Michigan State University
This CURE lab project is based on the research data published in 2018 from Dr. Christoph Benning's lab at Michigan State University. Overexpression of a plastid lipase gene PLIP3 in Arabidopsis resulted in severely reduced plant growth, accumulation of anthocyanin in plant tissues, and active jasmonate production, which is a fatty acid-based defense hormone protecting plants against pathogen attack. Several organelles participate in the biosynthesis of the hormone and we would like to understand how its biochemical precursors cross the membranes, especially the chloroplast membranes. The research question is: "What transporters are involved in moving jasmonate precursors across membranes during jasmonate biosynthesis?" Towards this end, we propose to conduct a suppressor screen that identifies genes that disrupt enzymes or transport proteins required for the biosynthesis of the hormone. In addition, receptors or signaling components mediating the hormone responses can be identified. The goal of this CURE project is to isolate and characterize such suppressor mutant(s) and identify those that disrupt the respective transporter genes.

Determining the Antioxidant Properties of Fruit
JACQUELINE SMITH, Bowie State University