An Arabidopsis Mutant Screen CURE for a Cell and Molecular Biology Laboratory Course part of CUREnet:CURE Collection
This CURE is designed from a crucial component of a chloroplast lipid signaling research project and has been implemented for a cell and molecular biology laboratory course at Michigan State University. The research laboratory generated an engineered plant line producing a lipid-derived plant hormone and mutagenized this line. The research question is "what transporters or receptors are involved in the hormone signaling transduction or perception processes?". Students form research hypotheses based on the research model, design experiments, perform experiments, collect and analyze data, make scientific arguments, and share their findings with the learning community. Specifically, the students culture the mutagenized plant population and select the desired mutant phenotypes, followed by genotyping the mutants and characterizing the mutants by basic biochemical approaches. Mathematics is also integrated into the course design. As the students studied the relevant genetic, molecular and biochemical concepts during this CURE, they use the core idea of information flow and data they generate in the lab to make claims about their mutant plants and support these claims with evidence and reasoning.

MCC: Malate Dehydrogenase CUREs Community part of CUREnet:CURE Collection
The Malate Dehydrogenase CUREs Community (MCC) project is designed to facilitate the adoption of effective, protein‐centric, Course Based Undergraduate Research Experiences (CUREs) into teaching labs at a wide variety of undergraduate serving institutions. (Primarily Undergraduate Institutions, Research Intensive Universities and Community Colleges) MCC coordinates and conducts pedagogical research into two major features of CUREs:1) their duration (whole semester versus 5‐6 week modules incorporated into a lab class), and 2) the impact of scientific collaboration between institutions (a key aspect of much modern research). Using validated assessment tools we seek to establish their effects on student confidence, persistence in STEM, and ability to design research experiments and interprete data. To facilitate faculty adoption of CURE approaches the project provides a number of resources. These focus on a variety of research areas related to Malate Dehydrogenase including mechanisms of catalysis and regulation, adaptation and evolution, cofactor specificity, folding and stability and interactions in metabolons. Resources include biologics, experimental protocols and assessment tools. The project also coordinates interactions between courses at different institutions to allow incorporation of scientific collaboration into CUREs. These collaborations also facilitate the use of more sophisticated experimental approaches and broaden the experimental scope of the CUREs.

Genomics Education Partnership part of CUREnet:CURE Collection
The goal of the Genomics Education Partnership is to provide opportunities for undergraduate students to participate in genomics research. GEP is a collaboration between a growing number of primarily undergraduate institutions, the Biology Dept and Genome Center of Washington University in St. Louis, and the Biology Dept at the University of Alabama. Participating undergraduates learn to take raw sequence data to high quality finished sequence, and to annotate genes and other features, leading to analysis of a question in genomics and research publication. GEP organizes research projects and provides training/collaboration workshops for PUI faculty and teaching assistants.

The HICA project part of CUREnet:CURE Collection
In this CURE, inspired by the work of Hoffmann, et al., students prepare mutant Haemophilus influenzae carbonic anhydrase (HICA) proteins. Using PyMOL to visualize the three-dimensional structure of the HICA protein, students choose one or more surface amino acid residues to mutate to histidine residues in order to create a surface histidine cluster that will allow the mutant protein to bind to a nickel affinity column. Using site-directed mutagenesis, recombinant plasmids are constructed and are then used to transform an E. coli expression vector. The mutant HICA protein is overexpressed, cells are lysed, and students load the cell lysate onto Ni-NTA columns and determine the imidazole concentration required to elute the mutant protein. The construction of a library of mutant proteins will allow the development of a general method in which specific surface histidine residues of any protein can be mutated in order to facilitate affinity purification. The Haemophilus influenzae bacterium described herein is a respiratory pathogen that causes meningitis (in its encapsulated form) and mucosal infections such as otitis media, sinusitis and conjunctivitis (in its unencapsulated form). A recent study showed that the carbonic anhydrase enzyme is absolutely required for pathogenesis. Furthermore, expression of the HICA enzyme allows the pathogen to survive in host immune cells (Langereis, et al.). These observations make the study of HICA itself particularly attractive, in addition to the overall goal of contributing to a body of work that will allow the minimal histidine character required for nickel affinity to be ascertained.

Problem Solving for Middle School Teachers part of CUREnet:CURE Collection
The primary goal of this class is for the students to creatively engage in mathematical investigations which provide the opportunity to integrate and apply previously learned mathematical knowledge to non-routine problems. We will examine a variety of strategies and skills that can be used to explore the fascinating world of mathematical problem solving. The course will cultivate students' habits of mind with respect to dissecting challenging mathematical problems for the purpose of solving them, as well as extending them by posing new related problems. Students will be expected to justify their solution methods through both written and oral presentations, demonstrating knowledge of reasoning and proof, mathematical communication, and mathematical connections. Each student will be expected to take an active role in all parts of the class. The assignments and activities are designed to enhance your understanding, appreciation, and practical experience of exploring and solving problems.

Global Change Microbiology part of CUREnet:CURE Collection
The dramatic impacts of human activities on Earth have catapulted the development of new disciplines across the sciences, humanities, and more. Studying the basis, challenges and responses to the global changes our planet and the human society face has become urgent. In the Global Change Microbiology CURE, students develop semester-long research projects focused on microbial communities and their relationship with a local environmental problem. Students: 1) develop research questions and conduct both field and wet lab work to estimate environmental, cell count and DNA-based diversity metrics; 2) receive training in bioinformatics, data analysis and result presentation; and 3) discuss literature on the interplay between microbes and environmental issues (e.g., global warming, ocean acidification, deoxygenation of coastal waters), the impacts of global changes on microbe-host interactions (e.g., coral bleaching, spreading of infectious diseases) and microbial applications (e.g., bioremediation, waste management). We examine key players in the whole spectrum of microorganisms (from viruses to microscopic animals), with emphasis on often overlooked protists that influence biogeochemical cycles, ecological functioning and host wellbeing.

The Art of Microbiology: an Agar Art Microbiology Lab CURE part of CUREnet:CURE Collection
Students use agar art made with freshly isolated microbes as a source for developing their own novel research projects.