Isolation and characterization of antibiotic-producing soil bacteria part of CUREnet:Institutes:NC Central University:Examples
One of the biggest threat in hospitals is the rising cases of people who harbor antibiotic-resistant bacterial strains. Therefore, it is critical to find and characterize novel antibiotics to combat the resistant strains. Most of the antibiotics used in healthcare settings come from anti-biotic producing bacteria and fungi found in the soil. The goal of this CURE will be to isolate antibiotic-producing bacteria and fungi from the soil in the local area, and to determine the chemistry of the antibiotics. An extension of the project will be to determine how the presence of antibiotic-producing microbes affect other organisms resident in the soil, as it is unclear as to why microbes use energy to produce antibiotic factors.

Neurogenetics Laboratory: Mapping a functional circuit for cold nociception in Drosophila part of CUREnet:Institutes:Alabama State University:Examples
Students will work in small groups to identify neural populations that may be involved in the Drosophila larval response to noxious cold. They will use the GAL4/UAS expression system to excite or inhibit neural populations and assess the impact of their manipulation on the larvae's behavioral response to cold. If a relevant neural population is identified, students will then identify (based on current literature) genes that are likely to be involved in neurite development and/or maintenance in that population. They will use mutations and/or RNA interference to disrupt the function of these genes in the population of interest and assess the effect of the disruption on neuronal morphology and larval behavior.

Resequencing of Commercial Microorganisms part of CUREnet:Institutes:Community College of Rhode island:Examples
Students choose a probiotic pill or product with labeling that indicates the species and strain of bacteria in the product. Products are chosen so that a high quality reference genome sequence is available on NCBI. After DNA isolation and library preparation, high-quality student samples are pooled for next-gen sequencing on an Illumina MiSeq. The following semester, students in the required bioinformatics course will analyze the FASTQ files from the NGS run with a simple variant call workflow on usegalaxy.org. Then, each student will use a R Shiny app developed for this CURE to convert the VCF output from Galaxy to a FASTA file for an assigned gene in the resequenced genome. Students will complete their research experience by submitting the FASTA file to the NCBI Nucleotide Database.

The Effect of Silver Nanoparticles on Plant Growth and Herbivory part of CUREnet:Institutes:Community College of Rhode island:Examples
In this CURE, students will conduct experiments to determine the effects of silver nanoparticles on plant growth and insect herbivory. Students will synthesize their own nanoparticles and treat Arabididopsis plants with them. After 5 weeks, insects (Pieris rapae, caterpillars) will be placed on plants and insect herbivory will be assessed across treatments. Insects will be weighted before and after feeding assays. Plant growth rates and insect herbivory measurements will be done using digital photography and image analysis using MathLab.

Exploring eukaryotic protein structure and post-translational modifications. part of CUREnet:Institutes:Bowie State University:Examples
This CURE will provide opportunity for students to think and act as researchers by using computational, biochemical, and bioanalytical techniques to examine tick antigen proteins. The CURE is designed as a lab for upper-level students who are taking or have taken a one-semester introductory biochemistry course, but two semesters would be even better. It could also be adapted for cell/molecular biology or (bio) analytical chemistry instrumentational analysis labs. It has been taught for classes ranging from 12-24 students. Ticks are notorious vectors of viral, protozoan, and bacterial diseases, including Lyme disease. While an anti-vector vaccine capable of protecting people from diseases transmitted by a particular tick species is an alluring goal, only one such anti-tick vaccine is currently available. This vaccine targets Bm86, a protein from the midgut of Rhipicephalus microplus, a cattle tick. Not only does the vaccine limit parasitism of the cattle by ticks, data suggests that it can also prevent transmission of tick-borne diseases including bovine anaplasmosis and babesiosis. However, similar vaccination approaches have not succeeded thus far against ticks that transmit diseases to humans, and little is known about the antibody response to the antigen, or about the protein itself. Since the protein's structure and function are unknown, the research goal of this CURE is to purify Bm86 using an insect cell/baculovirus expression system and characterize it, including domain structure and post-translational modifications (glycosylation sites). There are homologs to Bm86 in every sequenced tick species examined, and future CUREs will characterize some of the homologs including those in Ixodes scapularis, the tick that is mainly responsible for transmitting Lyme in the eastern US, and Haemaphysalis longicornis, the Asian longhorned tick, a newly-discovered invasive species in the area that also has significant disease-transmitting potential. By understanding the structure and post-translational modifications of this protein, we hope to gain a better understanding of how to make effective anti-tick vaccines, including those for humans, that may prevent transmission of Lyme disease. Importantly, the basic parameters of this CURE can be used to examine other proteins besides tick antigens. For example, during the pandemic, the CURE pivoted from the tick antigen to the SARS-CoV-2 nucleocapsid protein, which was also expressed in an insect cell system. Instead of characterizing glycosylation sites, we characterized phosphorylation sites. It's therefore possible to use this same framework for many different eukaryotic proteins that may be of research interest.

Worms Rule- Investigating variation in isoform function part of CUREnet:Institutes:Hampton University:Hampton CURE Examples
Integrating research into undergraduate science courses has been a long-term goal of many institutions. Research-based laboratory courses provide students with authentic research experiences while also helping them develop their analytical thinking and problem solving skills. Through these type of courses, students begin to understand and apply many fundamental concepts in biology while also contributing to the scientific field. To provide a research experience consisting of many common laboratory skills and the current buzz technique of CRISPR/Cas9 endogenous genome editing, we designed a one-semester research experience for undergraduates. By the end of a single semester, students enrolled in our upper level biology elective course successfully edited the genome of the nematode Caenorhabditis elegans (C. elegans). Throughout this course, students were exposed to molecular biology techniques (PCR, gel electrophoresis), imaging techniques (confocal microscopy), and CRISPR/Cas9 concept and techniques in C. elegans. Ultimately, the goal of this course was to provide students with a meaningful undergraduate research experience while generating reagents (namely C. elegans strains) that assist the instructor's personal research objectives.

Characterizing the Aging Process Using Caenorhabditis elegans and Reverse Genetics part of CUREnet:Institutes:Community College of Rhode island:Examples
Using gene silencing (RNAi) in the nemotode C. elegans, students will identify genetic modifiers of proteins with roles in aging by reverse genetics. Specifically, students will analyze the effect of knocking down genes on the level of aging-related proteins tagged with fluorophores (GFP, RFP, etc.). Each group of students will use function-specific RNAi libraries (transcription factors, kinases, etc) already established in our lab. Furthermore, students will evaluate the effect of genetic modifiers on proteostasis and lifespan. In addition to becoming familiar with C. elegans work and appreciating the use of model organisms, the students will master microscopy, genetic crosses, gene silencing, and molecular and biochemical readout assays such as qPCR and immunoblotting.