Tools and Data

Tool 1


ImageJ is a public domain Java image processing program. It runs, either as an online applet or as a downloadable application, on any computer with a Java 1.1 or later virtual machine. ImageJ can display, edit, analyze, process, save and print 8-bit, 16-bit and 32-bit images. It can read many image formats including TIFF, GIF and JPEG. It can "stack" a series of images that share a single window. It can calculate area and pixel value statistics of user-defined selections. It can measure distances and angles. Images can be zoomed up to 32:1 and down to 1:32. All analysis and processing functions are available at any magnification factor. Spatial calibration can be set to provide real world dimensional measurements.

Tool Builder

National Institutes of Health (NIH)

Tool Cost

Free: This software is in the public domain.

Tool Help

Tool 2

Microsoft Excel

A spreadsheet application is needed to analyze the data. Microsoft Excel is used in this chapter and is available as a stand-alone program or as part of Microsoft Office.

Tool Builder

Tool Cost

Students and educators may be able to purchase this software at a reduced cost.

Data Source

Total Ozone Mapping Spectrometer