An Arabidopsis Mutant Screen CURE for a Cell and Molecular Biology Laboratory Course
Jinjie Liu, Michigan State University
This CURE is designed from a crucial component of a chloroplast lipid signaling research project and has been implemented for a cell and molecular biology laboratory course at Michigan State University. The research laboratory generated an engineered plant line producing a lipid-derived plant hormone and mutagenized this line. The research question is "what transporters or receptors are involved in the hormone signaling transduction or perception processes?". Students form research hypotheses based on the research model, design experiments, perform experiments, collect and analyze data, make scientific arguments, and share their findings with the learning community. Specifically, the students culture the mutagenized plant population and select the desired mutant phenotypes, followed by genotyping the mutants and characterizing the mutants by basic biochemical approaches. Mathematics is also integrated into the course design. As the students studied the relevant genetic, molecular and biochemical concepts during this CURE, they use the core idea of information flow and data they generate in the lab to make claims about their mutant plants and support these claims with evidence and reasoning.

Synthesis of the Intermediate of a Catalytic Reaction: An NHC-Stabilized, First-Row Transition Metal Complex
Meng Zhou, Lawrence Technological University
The advanced synthesis laboratory course object allows students to study the synthesis, purification, and characterizations of a new diamagnetic organometallic complex of a first-row transition metal. The air-stable complex is stabilized by an N-heterocyclic carbene spectator ligand. It also bears an actor ligand and therefore, is potentially a reactive intermediate of a catalytic reaction. The synthesis of a reactive intermediate is the key to elucidate the mechanism of catalysis. The instructor chooses the first-row transition metal and the actor ligand based on his or her interests. The CURE starts from an NHC-ligated complex that does not bear this actor ligand but is otherwise similar. In our CURE, an anion ligand-replacement reaction was used to install the actor ligand, but an instructor may choose other approaches. The students will evaluate their results by standard spectroscopic analyses using UV-vis, FT-IR, and proton NMR (60 MHz or above) analysis.

Characterizing the Aging Process Using Caenorhabditis elegans and Reverse Genetics
Joslyn Mills, Bridgewater State University
Using gene silencing (RNAi) in the nemotode C. elegans, students will identify genetic modifiers of proteins with roles in aging by reverse genetics. Specifically, students will analyze the effect of knocking down genes on the level of aging-related proteins tagged with fluorophores (GFP, RFP, etc.). Each group of students will use function-specific RNAi libraries (transcription factors, kinases, etc) already established in our lab. Furthermore, students will evaluate the effect of genetic modifiers on proteostasis and lifespan. In addition to becoming familiar with C. elegans work and appreciating the use of model organisms, the students will master microscopy, genetic crosses, gene silencing, and molecular and biochemical readout assays such as qPCR and immunoblotting.

Biomass conversion into highly useful chemicals
SAPNA JAIN, Alabama State University
This is CURE based course that aims at bridging the gap between theoretical knowledge in chemistry and its practical applications at solving real-world problems. It gives students an opportunity to construct and synthesize their knowledge and skills by learning to apply theoretical knowledge to practice by the laboratory research. The purpose of this course is to acquaint students with the fundamental concepts of chemistry, synthetic methods and techniques. The emphasis will be on novel catalysts synthesis and evaluating their activity towards biomass conversion to liquid fuel and useful chemicals. Students will design synthesize, deduce identities of the biomass conversion products from chemical and spectral clues, and predict reaction products.

Population & Community Ecology
Cascade Sorte, University of California-Irvine
Students in a Population and Community Ecology class participate in coastal marine research focused on understanding factors determining population sizes and community interactions, particularly in the context of species that appear to be shifting their ranges with climate change. Students participate in all aspects of the research from making observations and collecting data in the field to defining questions, stating hypothesis, designing and completing statistical analysis, and interpreting and presenting results. The outcomes are a research proposal, research paper, and poster presentation. All are intended to be at a level appropriate for use as a writing sample or presentation at undergraduate conferences. Results are incorporated into the ongoing research project led by the course instructor and graduate student teaching assistant.

Molecular Parasitology
Swati Agrawal, University of Mary Washington
In Spring 2021, we piloted a mini-CURE where student groups from University of Mary Washington and Georgia State University collaboratively completed research projects as part of a research-intensive course on Molecular Parasitology. The benefits of this approach were immediately obvious as students interacted across institutions, learned from each other's disciplinary expertise while informing their own research with data collected by their collaborators.

Worms Rule- Investigating variation in isoform function
Anna Allen, Howard University
Integrating research into undergraduate science courses has been a long-term goal of many institutions. Research-based laboratory courses provide students with authentic research experiences while also helping them develop their analytical thinking and problem solving skills. Through these type of courses, students begin to understand and apply many fundamental concepts in biology while also contributing to the scientific field. To provide a research experience consisting of many common laboratory skills and the current buzz technique of CRISPR/Cas9 endogenous genome editing, we designed a one-semester research experience for undergraduates. By the end of a single semester, students enrolled in our upper level biology elective course successfully edited the genome of the nematode Caenorhabditis elegans (C. elegans). Throughout this course, students were exposed to molecular biology techniques (PCR, gel electrophoresis), imaging techniques (confocal microscopy), and CRISPR/Cas9 concept and techniques in C. elegans. Ultimately, the goal of this course was to provide students with a meaningful undergraduate research experience while generating reagents (namely C. elegans strains) that assist the instructor's personal research objectives.

Using NSF's NEON Data in an Undergraduate Ecology CURE on the Ecological Impacts of Global Climate Change
Jennifer Kovacs, Agnes Scott College
We live in a time where we can see a very real need for a basic understanding of ecological terminology, concepts, and methodologies to improve public policy and other ecological problem-solving decisions, especially in light of global climate change. Across the field, there is a major push to incorporate computational thinking and an understanding of human social systems throughout the science curriculum. In ecology and other STEMM fields, basic programming and coding skills have become essential and marketable, as has the ability to mine and analyze large data sets.In this semester-long CURE, students individually develop and answer their own ecological research question using a selection of publicly available datasets from the expansive NSF NEON data repository. Generally, at the beginning of the course the instructor selects several data products from a specific geographic region. After gaining familiarity with the NEON project through videos, a NEON data tutorial, and a case study, students also use these curated NEON data products to begin forming their independent research projects. Most students ultimately incorporate other data products either from NEON or other databases into their final research projects. Students use mostly R to download, wrangle, and analyze their data. The instructor assumes no prior knowledge of R or coding at the beginning of the course. Throughout the semester, students complete mini-assignments and tutorials which introduce them to the necessary coding skills to download, clean, analyze, and visualize their chosen data products. Additionally, students are provided with a wide range of free resources, including videos, tutorials, and the free online textbook Passion Driven Statistics to help them master the skills they need to complete their individual research projects. During weekly in-class one-on-one meetings with the instructor, students work to identify, collect, and analyze data that would address an existing hypothesis/ problem in the field of ecology and global climate change. Ultimately, students present their findings to the larger campus community during the annual undergraduate research day at our institution.

Exploring the Structure-Function Relationship in RNA Biochemistry

Isolation and characterization of antibiotic-producing soil bacteria
Maria Messner, Lenoir Community College
One of the biggest threat in hospitals is the rising cases of people who harbor antibiotic-resistant bacterial strains. Therefore, it is critical to find and characterize novel antibiotics to combat the resistant strains. Most of the antibiotics used in healthcare settings come from anti-biotic producing bacteria and fungi found in the soil. The goal of this CURE will be to isolate antibiotic-producing bacteria and fungi from the soil in the local area, and to determine the chemistry of the antibiotics. An extension of the project will be to determine how the presence of antibiotic-producing microbes affect other organisms resident in the soil, as it is unclear as to why microbes use energy to produce antibiotic factors.