Performing a BLAST Search

Questions you must be able to answer in order to perform a blast search:

  1. What sequence(s) are the subject of my research? These are called query sequences. Example: I am examining the potential roles of the 10 most abundant sequences in the Chamaecrista sequence dataset. I will use these as the query sequences in a blast search.
  2. What subject database is the most appropriate to search, given my research question?
blast algorithms

How to perform a blast search:

Step 1: Retrieve a query sequence you want to use and make sure it is in fasta format
Step 2: Navigate to the database you want to search against
Step 3: Select the BLAST programthat will use the query sequence to search the database that you selected.
Step 4: Adjust any parameters, as appropriate