A new instrument was devised to identify misconceptions in biology and probe into the causes of misunderstanding in a relatively quick way. It revealed that novice biology teachers held a number of conceptual errors which were prevalent among secondary school students. They showed serious misunderstandings in cellular metabolism, the nutritional process, gaseous exchange, the circulatory system, homeostasis, reproduction and variation. The problem has been attributed to inadequate mastery of subject knowledge and imprecise use of terminology. Specific teaching strategies were suggested to prevent the propagation of these misconceptions in students. Teacher education programmes should aim at promoting the awareness of teachers as to children's learning difficulties and developing their professional skills in facilitating children's conceptual development.