Using Ion Exchange Chromatography to Separate Proteins

Ellyn A. Daugherty, Access Excellence at the National Health Museum Activities Exchange

This activity website, hosted by the National Health Museum Activities Exchange, explores protein purification using ion chromatography. Students will separate a positively charged lysozyme from a negatively charged albumin at a neutral pH using ion exchange chromatography. The site includes information for the teacher, instructions and follow-up questions for students, and a list of supplies.

This resource is referenced here:
Subject: Biology, Biogeochemistry, Microbiology, Geoscience:Geology:Geochemistry:Organic and Biochemistry
Resource Type: Activities:Lab Activity
Grade Level: College Upper (15-16), College Lower (13-14), High School (9-12)
Theme: Teach the Earth:Course Topics:Geochemistry, BiogeoscienceKeywords: anion, cation, column chromatography