Denaturing Gradient Gel Electrophoresis Profiles of 16S rRNA-Defined Populations Inhabiting a Hot Spring Microbial Mat Community

MJ Ferris, G Muyzer, DM Ward, Applied and Environmental Microbiology

This journal abstract reports population profiles of different temperature regions in the microbial mat community of Octopus Spring, Yellowstone National Park based on denaturing gradient gel electrophoresis (DGGE) analyses. Profiles were essentially identical within regions of the mat defined by one temperature range but varied between sites with different temperature ranges. Cyanobacteria, green nonsulfur bacterium-like, and green sulfur bacterium-like populations were detected as well as a novel bacterial population of uncertain phylogenetic affiliation. A link to the full article in PDF format is provided.

Subject: Biology:Diversity, Ecology:Biofilms, Symbiotic Relations:Mutualism, Biology:Ecology:Principles, Biology:Biogeochemistry, Microbiology:Methods of Microbiology :Molecular Methods, Biology:Microbiology, Molecular Biology
Resource Type: Pedagogic Resources:Research Results
Grade Level: Graduate/Professional, College Upper (15-16)
Extreme Environments: Extremely Hot
Topics: Biosphere:Diversity, Ecology:Biofilms, Abiotic Relations, Biosphere:Biogeochemistry, Molecular Biology, Methods of Microbiology:Molecular Methods , Biosphere:Microbiology, Ecology:Principles
Theme: Teach the Earth:Course Topics:BiogeoscienceKeywords: hot spring, PCR, distribution