Seasonal Distributions of Dominant 16S rRNA-Defined Populations in a Hot Spring Microbial Mat Examined by Denaturing Gradient Gel Electrophoresis

MJ Ferris, DM Ward, Applied and Environmental Microbiology

This journal abstract reports the distributions of bacterial populations within a microbial mat of Octopus Spring in Yellowstone National Park. Sites along the thermal gradient of the spring's effluent channel were surveyed at seasonal intervals by denaturing gradient gel electorphoresis (DGGE) analysis of PCR-amplified 16S rRNA gene segments. A new cyanobacterial 16S rRNA sequence type and a new green nonsulfur bacterium-like sequence type were detected in this study. A link to the full article in PDF format is provided.

Subject: Biology:Ecology:Biofilms, Principles, Biology:Diversity, Molecular Biology, Microbiology:Methods of Microbiology :Molecular Methods, Biology:Microbiology
Resource Type: Scientific Resources:Research Results
Grade Level: Graduate/Professional, College Upper (15-16)
Extreme Environments: Extremely Hot
Topics: Biosphere:Diversity, Ecology:Biofilms, Biosphere:Molecular Biology, Microbiology, Ecology:Principles, Biosphere:Methods of Microbiology:Molecular Methods Keyword: hot spring