Anna-Louise Reysenbach, Gene S. Wickham, Norman R. Pace, Applied and Environmental Microbiology
This journal article reports the molecular phylogenetic approach used to analyze a microbial community associated with the 84 to 88 degrees C outflow from Octopus Spring. The authors perform techniques such as microscopy, DNA extraction and amplification, cloning and sequence analysis of 16S rRNA genes, phylogenetic analysis, and in situ hybridization to determine the limited phylogenetic diversity of the pink filaments – including hydrogen-oxidizing bacteria. Located in PubMed, this article contains a link to a printable PDF version.
Subject: Biology:Evolution:Patterns, Biology:Diversity, Biogeochemistry, Molecular Biology, Microbiology, Microbiology:Methods of Microbiology :Molecular Methods, Microscopy Resource Type: Scientific Resources:Research Results Grade Level: College Upper (15-16), Graduate/Professional Extreme Environments: Extremely Hot Theme: Teach the Earth:Course Topics:BiogeoscienceKeywords: Aquifex pyrophilus, Hydrogenobacter thermophilus, Thermotogales
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