Phylogenetic Analysis of the Hyperthermophilic Pink Filament Community in Octopus Spring, Yellowstone National Park

Anna-Louise Reysenbach, Gene S. Wickham, Norman R. Pace, Applied and Environmental Microbiology

This journal article reports the molecular phylogenetic approach used to analyze a microbial community associated with the 84 to 88 degrees C outflow from Octopus Spring. The authors perform techniques such as microscopy, DNA extraction and amplification, cloning and sequence analysis of 16S rRNA genes, phylogenetic analysis, and in situ hybridization to determine the limited phylogenetic diversity of the pink filaments including hydrogen-oxidizing bacteria. Located in PubMed, this article contains a link to a printable PDF version.

Subject: Biology:Biogeochemistry, Diversity, Molecular Biology, Microbiology, Microbiology:Methods of Microbiology :Microscopy, Molecular Methods, Biology:Evolution:Patterns
Resource Type: Scientific Resources:Research Results
Grade Level: Graduate/Professional, College Upper (15-16)
Extreme Environments: Extremely Hot
Topics: Biosphere:Biogeochemistry, Molecular Biology, Diversity, Methods of Microbiology:Molecular Methods , Microscopy , Biosphere:Evolution:Patterns, Biosphere:Microbiology
Theme: Teach the Earth:Course Topics:BiogeoscienceKeywords: Aquifex pyrophilus, Hydrogenobacter thermophilus, Thermotogales