Microbial Life > Topics of Interest > Lake Pontchartrain > Lake Pontchartrain: Sediment Sampling Activity

Lake Pontchartrain: Sediment Sampling

Created by David Patterson & Sarah Bordenstein, Marine Biological Laboratory

In this activity, students will:

  • Learn how to collect microbes from sediment samples
  • Use a microscope to observe and record major groups of environmental microorganisms
Materials - Procedure - Resources - Standards

Materials Needed


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Procedure


Day 1: Prepare Environmental Sample
  1. Collect mud or sand samples from Lake Pontchartrain in an area where the water is about 1 foot deep. Collect just the surface sediments (black sediments are bad!). Place samples in bucket and layer with about an inch of water.
  2. Transport samples to the classroom and sieve onto shallow dishes - such as disposable plastic food containers.
  3. Leave the sample for 10 minutes. Using a disposable pipette, remove water from the top of the samples to a beaker until the sediment is damp but with no sloshing water. (The excess water may later be used to make a wet mount.)
  4. Place one sheet of lens paper directly on top of the samples and put about six cover slips (22 X 22 mm No 1's are best) on top of each lens paper. Place a lid loosely on top of the container. Over the next two or three days, bottom of the sediments will become anoxic and the microorganisms will migrate from the sediment, through the lens paper, and up to the coverslip. You can then lift the coverslips carefully off the sediment to look at the mnicrobial community that has accumulated.
Day 3 or 4: Make a Wet Mount
  1. Using ridged forceps, gently lift a coverslip from the top of the sediment. The coverslip should already be wet enough to prepare a wet mount. If it is too dry, add a drop of excess water from the sample onto the microscope slide.
  2. While holding the coverslip upright, carefully touch one edge on the slide.
  3. Trying to minimize the amount of air bubbles under the coverslip, slowly and gently lower it facedown onto the slide. (Hint: if your slide is too wet, the coverslip will fall off when you tilt it horizontally.)
  4. Put the slide on a microscope, and starting with lowest magnifications to help orient yourself, navigate around to find as many kinds of microbes as you can (e.g., bacteria, diatoms, ciliates, and amoebae).
  5. Make sketchs of the organisms observed. If available, use videos or cameras to create 'uninterpreted' records of what you see.
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Resources


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Louisiana Science Content Standards, 9-12


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