Isi Ero-Tolliver

DNA cloning and protein analysis of animal-heme peroxidase within collagen IV of the extracellular matrix part of CUREnet:Institutes:Hampton University:Hampton CURE Examples
This CURE is to expose undergraduate students to the process of DNA cloning to identify the critical amino acids of the animal-heme peroxidase,peroxidasin, responsible for catalyzing sulfilimine bond formation within collagen IV of the basement membrane. Students will bioengineer a variety of mutants through primer design and polymerase chain reactions that contain point mutations within the immunoglobulin domain of the peroxidasin.