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This online educational module analyzes reproductive isolation as a cause of speciation. It offers a definition of the term as well as specific examples of barriers to gene flow that may contribute to speciation, including evolution of different mating location, mating time or mating rituals, lack of &quot;fit&quot; between sexual organs, and offspring inviability or sterility. The webpage is part of the Understanding Evolution educational website, a noncommercial resource designed to help you understand what evolution is, how it works, how it factors into your life, how research in evolutionary biology is performed, and how ideas in this area have changed over time.