Anne K. Dunn, Deborah S. Millikan, Dawn M. Adin, Jeffrey L. Bose, Eric V. Stabb, Applied and Environmental Microbiology
This AEM scholarly article examines two limitations to studying the in situ symbiosis of Euprymna scolopes, or bobtail squid, and Vibrio fischeri. Current molecular studies of this symbiosis lack plasmids that maintain stability in the absence of antibiotic pressure and need a useful fluorescent tag that can be used with green fluorescent protein (GFP), which holds great promise as an in situ reporter of gene activity. The authors introduce a possible remedy to these limitations by utilizing plasmid pES213-based vectors and Red Fluorescent Protein (RFP) – a visual tag that could be used alongside GFP.