What a couple of dimensions can do for you: Comparative developmental studies using 4D microscopy, examples from tardigrade development
http://icb.oxfordjournals.org/cgi/rapidpdf/icj012v2.pdf

Andreas Hejnol1, Ralf Schnabel, Integrative and Comparative Biology


This Integrative and Comparative Biology scholarly paper (11-page PDF) presents a sophisticated method of microscopy, 4D microscopy (3D time-lapse microscopy), which records the entire development of an embryo and allows detailed analyses of events such as cleavage, cell migration, cell death (apoptosis), and cell differentiation during development. Using tardigrade development as an example, this paper demonstrates that the use of 4D microscopy can reveal new characters and corroborate or disapprove old characters. It also discusses results in the light of recent phylogenetic hypotheses regarding the Arthropoda and their probable sister group, the Cycloneuralia, which together form the Ecdysozoa.

This resource is referenced here:
Subject: Biology:Microbiology, Microbiology:Methods of Microbiology :Microscopy
Resource Type: Scientific Resources:Research Results
Grade Level: Graduate/Professional, College Lower (13-14), College Upper (15-16)
Topics: Biosphere:Methods of Microbiology:Microscopy , Biosphere:MicrobiologyKeywords: water bears, Tardigrada