Phylogenetic Analysis of the Hyperthermophilic Pink Filament Community in Octopus Spring, Yellowstone National Park
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=201609

Anna-Louise Reysenbach, Gene S. Wickham, Norman R. Pace, Applied and Environmental Microbiology


This journal article reports the molecular phylogenetic approach used to analyze a microbial community associated with the 84 to 88 degrees C outflow from Octopus Spring. The authors perform techniques such as microscopy, DNA extraction and amplification, cloning and sequence analysis of 16S rRNA genes, phylogenetic analysis, and in situ hybridization to determine the limited phylogenetic diversity of the pink filaments including hydrogen-oxidizing bacteria. Located in PubMed, this article contains a link to a printable PDF version.


Subject: Biology:Biogeochemistry, Diversity, Molecular Biology, Microbiology, Microbiology:Methods of Microbiology :Microscopy, Molecular Methods, Biology:Evolution:Patterns
Resource Type: Scientific Resources:Research Results
Grade Level: Graduate/Professional, College Upper (15-16)
Theme: Teach the Earth:Course Topics:Biogeoscience
Extreme Environments: Extremely Hot
Topics: Biosphere:Biogeochemistry, Diversity, Molecular Biology, Methods of Microbiology:Molecular Methods , Microscopy , Biosphere:Evolution:Patterns, Biosphere:MicrobiologyKeywords: Aquifex pyrophilus, Hydrogenobacter thermophilus, Thermotogales