Teaching Genomics at Small Colleges > Inquiry-based Integrated Instructional Units > SNP Lab Materials, Supplies, and Set Up

Supplies and Equipment Needed for "Human SNP Determination" Lab Activity


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For the first lab session, "DNA Isolation and PCR"

For students in each lab section (20-24 students) to share:

Per lab group (2-3 students):

Aliquots for each group of 3 students:

Other aliquots:

Ordering and preparing primers:

We order the following two primers for this lab (from Operon Biotechnologies):

We then dilute each of these to 5 micromolar stocks. For the lab, we mix the primers together with sterile water so that each primer has a concentration of 0.2083 micromolar. This means that the primers will be at a final concentration of 0.2 micromolar when we mix it with the DNA in the PCR tube.

For the second lab session, "Restriction Enzyme Digest and Gel Electrophoresis"

For students in each lab section (20-24 students) to share:

Per lab group (2-3 students):

Aliquots for each group of 3 students:


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